endogenous cx43  (ATCC)

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: The antibodies used in this study with concentrations used and their source companies.

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques: Immunofluorescence

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: Endogenous connexin expression in right atrial muscle. (A) Illustrative membranes from western blotting are shown for the connexin proteins: Cx45 (single band at 45 kDa), Cx43 doublet (band at 43 kDa is phosphorylated Cx43, the band at 41 kDa is non-phosphorylated Cx43) and Cx40 (single band at 40 kDa). Equal protein-loading was confirmed by desmin labelling (53 kDa). (B) Total endogenous Cx43 protein (μg) per total protein (μg) significantly declined with ageing ( n = 5; ANOVA p < 0.001).

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques: Expressing, Western Blot

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: Dimensions of the intercalated disc. Myocyte width (A) and intercalated disc area (B) significantly increased between age groups ( n = 30; ANOVA p < 0.001). Intensity of total endogenous Cx43 protein from each intercalated disc expressed as a percentage of the total disc area (C) was found to decline with age ( n = 50; ANOVA p < 0.0001; linear regression, y = −0.94x + 55.4).

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques:

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: Example projection confocal images of intercalated discs with Cx43 labelled from animals of 1 month and 38 months of age (A). For the position indicated by the dashed line that bisects each disc image (A) an example intensity profile of Cx43 distribution across the intercalated disc from animals at (B) 1 month and (C) 38 months of age is shown.

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques:

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: The representative membrane (A) illustrates the changes in phosphorylated Cx43 (P-Cx43) with age, expressed as the ratio of P-Cx43 to total Cx43 protein. (B) Illustrates the changes in activated-JNK with age, expressed as a ratio of activated JNK to non-activated JNK (NA-JNK). (A–B) Desmin content is shown to verify equal protein loading. (C) Shows the correlation between the ratio of proteins activated-JNK:NA-JNK and P-Cx43:total Cx43 across the age range ( n = 5 per age group; ANOVA p < 0.01; linear regression y = 1.0305X–0.3484 = 0.96). (D) p38 (upper blot) and phosphorylated p38 (lower blot) at each age with average data shown below (mean ± SD). The dashed lines show linear regressions to the data. There is no significant correlation between p38 or phosphorylated p38, and age ( R 2 = 0.03 and 0.42, respectively, by Pearson correlation).

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques: Membrane

Journal: Mechanisms of Ageing and Development

Article Title: Progressive age-associated activation of JNK associates with conduction disruption in the aged atrium

doi: 10.1016/j.mad.2015.05.001

Figure Lengend Snippet: Isolated single cardiac myocytes were either treated with vehicle only (DMSO, control), exposed to the pharmacological agent SP600125 (JNK inhibitor) or anisomycin (an activator of JNK) an example membrane from western blot (A) illustrates the associated changes in Cx43 and JNK (desmin levels verify equal protein loading). Exposure to anisomycin increased activated-JNK (expressed as a ratio of activated-JNK to non-activated JNK) but SP600125 eliminated all trace of activated-JNK. Cx43 protein expression significantly declines in both the presence of anisomycin and SP600125 in comparison with the untreated myocytes (A) ( n = 10; ANOVA, p < 0.01). The ‘untreated’ myocytes exposed to DMSO alone show Cx43 at the intercalated discs (B) ( n = 20 per group). Myocytes exposed to anisomycin had significantly less Cx43 at the intercalated discs and SP600125 further reduced Cx43 labelling to visually undetectable levels ( n = 20 per group, ‡ p < 0.0001). The red labelling is from wheat germ agglutinin included as a membrane marker.

Article Snippet: Total endogenous Cx43 , Cell signalling Tech., Hertfordshire, UK , Rabbit , 1 , 0.1.

Techniques: Isolation, Control, Membrane, Western Blot, Expressing, Comparison, Marker